› 4kk0

X-ray diffraction
2.9Å resolution

Crystal Structure of TSC1 core domain from S. pombe

Released:
DOI: 10.2210/pdb4kk0/pdb
PDB entry 4kk0 coloured by chain, front view.
PDB entry 4kk0 coloured by chain, side view.
PDB entry 4kk0 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Crystal structure of the yeast TSC1 core domain and implications for tuberous sclerosis pathological mutations.
Sun W, Zhu YJ, Wang Z, Zhong Q, Gao F, Lou J, Gong W, Xu W
Nat Commun 4 2135 (2013)
PMID: 23857276

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tuberous sclerosis 1 protein homolog Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 461 amino acids
Theoretical weight: 53.74 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09778 (Residues: 1-431; Coverage: 48%)
Gene names: SPAC22F3.13, tsc1
Sequence domains: Hamartin protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 70 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21212
Unit cell:
a: 231.375Å b: 239.791Å c: 112.014Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.256
Expression system: Escherichia coli

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Citations

3 review citations

Regulation of mTORC1 by PI3K signaling.
Dibble et al. (2015)
2 more

1 mention without citation

Signal integration in the (m)TORC1 growth pathway.
Ramlaul et al. (2018)