› 4kmr

X-ray diffraction
1.45Å resolution

Structure of a putative transcriptional regulator of LacI family from Sanguibacter keddieii DSM 10542.

Released:
DOI: 10.2210/pdb4kmr/pdb
PDB entry 4kmr coloured by chain, front view.
PDB entry 4kmr coloured by chain, side view.
PDB entry 4kmr coloured by chain, top view.
Source organism: Sanguibacter keddieii DSM 10542
Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Endres M, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 29.97 KDa
Source organism: Sanguibacter keddieii DSM 10542
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1BJM2 (Residues: 66-345; Coverage: 81%)
Gene name: Sked_03100
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 42.175Å b: 120.205Å c: 44.962Å
α: 90° β: 92.59° γ: 90°
R-values:
R R work R free
0.128 0.125 0.178
Expression system: Escherichia coli BL21(DE3)

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