› 4knf

X-ray diffraction
2.6Å resolution

Crystal structure of a blue-light absorbing proteorhodopsin double-mutant D97N/Q105L from HOT75

Released:
DOI: 10.2210/pdb4knf/pdb
PDB entry 4knf coloured by chain, front view.
PDB entry 4knf coloured by chain, side view.
PDB entry 4knf coloured by chain, top view.
Source organism: gamma proteobacterium 'Hot 75m4'
Primary publication:
Cross-protomer interaction with the photoactive site in oligomeric proteorhodopsin complexes.
Ran T, Ozorowski G, Gao Y, Sineshchekov OA, Wang W, Spudich JL, Luecke H
Acta Crystallogr D Biol Crystallogr 69 1965-80 (2013)
PMID: 24100316

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Blue-light absorbing proteorhodopsin Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 261 amino acids
Theoretical weight: 28.1 KDa
Source organism: gamma proteobacterium 'Hot 75m4'
UniProt:
  • Canonical: Q9AFF7 (Residues: 1-251; Coverage: 100%)
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 5 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 161.4Å b: 168.9Å c: 65.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.267

Quick links

Citations

7 review citations

Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.
Govorunova et al. (2017)
6 more

3 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
2 more