› 4kpa

X-ray diffraction
2Å resolution

Crystal structure of cytochrome P450 BM-3 in complex with N-palmitoylglycine (NPG)

Released:
DOI: 10.2210/pdb4kpa/pdb
PDB entry 4kpa coloured by chain, front view.
PDB entry 4kpa coloured by chain, side view.
PDB entry 4kpa coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Structural evidence: a single charged residue affects substrate binding in cytochrome P450 BM-3.
Catalano J, Sadre-Bazzaz K, Amodeo GA, Tong L, McDermott A
Biochemistry 52 6807-15 (2013)
PMID: 23829560

Function and Biology Details

Reactions catalysed: 
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional cytochrome P450/NADPH--P450 reductase Chain: A
Molecule details ›
Chain: A
Length: 494 amino acids
Theoretical weight: 56.52 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P14779 (Residues: 1-471; Coverage: 45%)
Gene names: BG04_163, cyp102, cyp102A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand 140 1 x 140
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21212
Unit cell:
a: 188.7Å b: 59.32Å c: 56.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.23
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Bioorthogonal Enzymatic Activation of Caged Compounds.
Ritter et al. (2015)
6 more

3 mentions without citation

Plumage redness signals mitochondrial function in the house finch.
Hill et al. (2019)
2 more