› 4kpp

X-ray diffraction
2.3Å resolution

Crystal Structure of H+/Ca2+ Exchanger CAX

Released:
DOI: 10.2210/pdb4kpp/pdb
PDB entry 4kpp coloured by chain, front view.
PDB entry 4kpp coloured by chain, side view.
PDB entry 4kpp coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Primary publication:
Structural basis for the counter-transport mechanism of a H+/Ca2+ exchanger.
Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O
Science 341 168-72 (2013)
PMID: 23704374

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sodium/calcium exchanger membrane region domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 45.02 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O29988 (Residues: 1-394; Coverage: 100%)
Gene name: AF_0251
Sequence domains: Sodium/calcium exchanger protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand OLB 1 x OLB
Ligand OLA 5 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21
Unit cell:
a: 63.954Å b: 97.386Å c: 71.233Å
α: 90° β: 99.37° γ: 90°
R-values:
R R work R free
0.204 0.202 0.238
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Calcium transport across plant membranes: mechanisms and functions.
Demidchik et al. (2018)
14 more

4 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
3 more