› 4kpr

X-ray diffraction
2.4Å resolution

Tetrameric form of rat selenoprotein thioredoxin reductase 1

Released:
DOI: 10.2210/pdb4kpr/pdb
PDB entry 4kpr coloured by chain, front view.
PDB entry 4kpr coloured by chain, side view.
PDB entry 4kpr coloured by chain, top view.
Source organism: Rattus norvegicus
Entry authors: Lindqvist Y, Sandalova T, Xu J, Arner E

Function and Biology Details

Reactions catalysed: 
NADPH + H(2)O(2) = NADP(+) + 2 H(2)O
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin reductase 1, cytoplasmic Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 499 amino acids
Theoretical weight: 54.77 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O89049 (Residues: 1-499; Coverage: 100%)
Gene names: Trxr1, Txnrd1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
2 bound ligands:
Ligand SO3 3 x SO3
Ligand MPD 9 x MPD
1 modified residue:
Ligand SE7 4 x SE7

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3121
Unit cell:
a: 162.99Å b: 162.99Å c: 236.44Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.187
Expression system: Escherichia coli BL21(DE3)

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