› 4kpu

X-ray diffraction
1.6Å resolution

Electron transferring flavoprotein of Acidaminococcus fermentans: Towards a mechanism of flavin-based electron bifurcation

Released:
DOI: 10.2210/pdb4kpu/pdb
PDB entry 4kpu coloured by chain, front view.
PDB entry 4kpu coloured by chain, side view.
PDB entry 4kpu coloured by chain, top view.
Source organism: Acidaminococcus fermentans DSM 20731
Primary publication:
Studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) of Acidaminococcus fermentans.
Chowdhury NP, Mowafy AM, Demmer JK, Upadhyay V, Koelzer S, Jayamani E, Kahnt J, Hornung M, Demmer U, Ermler U, Buckel W
J Biol Chem 289 5145-57 (2014)
PMID: 24379410

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112403 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Electron transfer flavoprotein alpha/beta-subunit N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 37.93 KDa
Source organism: Acidaminococcus fermentans DSM 20731
Expression system: Escherichia coli
UniProt:
  • Canonical: D2RIQ3 (Residues: 1-340; Coverage: 100%)
Gene name: Acfer_0556
Sequence domains:
Structure domains:
Electron transfer flavoprotein small subunit Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 28.43 KDa
Source organism: Acidaminococcus fermentans DSM 20731
Expression system: Escherichia coli
UniProt:
  • Canonical: D2RIQ2 (Residues: 1-263; Coverage: 100%)
Gene name: Acfer_0555
Sequence domains: Electron transfer flavoprotein domain
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 80.18Å b: 84.89Å c: 106.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.191
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria.
Schuchmann et al. (2014)
10 more

11 mentions without citation

Distribution, Evolution, Catalytic Mechanism, and Physiological Functions of the Flavin-Based Electron-Bifurcating NADH-Dependent Reduced Ferredoxin: NADP+ Oxidoreductase.
Liang et al. (2019)
10 more