› 4kqe

X-ray diffraction
2.74Å resolution

The mutant structure of the human glycyl-tRNA synthetase E71G

Released:
DOI: 10.2210/pdb4kqe/pdb
PDB entry 4kqe coloured by chain, front view.
PDB entry 4kqe coloured by chain, side view.
PDB entry 4kqe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Large Conformational Changes of Insertion 3 in Human Glycyl-tRNA Synthetase (hGlyRS) during Catalysis.
Deng X, Qin X, Chen L, Jia Q, Zhang Y, Zhang Z, Lei D, Ren G, Zhou Z, Wang Z, Li Q, Xie W
J Biol Chem 291 5740-5752 (2016)
PMID: 26797133

Function and Biology Details

Reaction catalysed: 
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 693 amino acids
Theoretical weight: 78.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41250 (Residues: 55-739; Coverage: 93%)
Gene names: GARS, GARS1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 91.239Å b: 91.239Å c: 246.614Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.255
Expression system: Escherichia coli

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Citations

1 review citation

Drugging tRNA aminoacylation.
Ho et al. (2018)
10 other articles

5 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
4 more