› 4krt

X-ray diffraction
1.92Å resolution

X-ray structure of endolysin from clostridium perfringens phage phiSM101

Released:
DOI: 10.2210/pdb4krt/pdb
PDB entry 4krt coloured by chain, front view.
PDB entry 4krt coloured by chain, side view.
PDB entry 4krt coloured by chain, top view.
Source organism: Clostridium phage phiSM101
Primary publication:
X-ray structure of a novel endolysin encoded by episomal phage phiSM101 of Clostridium perfringens.
Tamai E, Yoshida H, Sekiya H, Nariya H, Miyata S, Okabe A, Kuwahara T, Maki J, Kamitori S
Mol Microbiol 92 326-37 (2014)
PMID: 24674022

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
lysozyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 40.07 KDa
Source organism: Clostridium phage phiSM101
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0SPG7 (Residues: 1-342; Coverage: 100%)
Gene name: CPR_C0050
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EPE 1 x EPE
Ligand ACT 14 x ACT
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 55.6Å b: 112.9Å c: 64.95Å
α: 90° β: 91.35° γ: 90°
R-values:
R R work R free
0.226 0.226 0.261
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Bacteriophage endolysins: applications for food safety.
Schmelcher et al. (2016)
6 more

4 mentions without citation

Surfaceome and Proteosurfaceome in Parietal Monoderm Bacteria: Focus on Protein Cell-Surface Display.
Desvaux et al. (2018)
3 more