› 4ksr

X-ray diffraction
4.2Å resolution

Crystal Structure of the Vibrio cholerae ATPase GspE Hexamer

Released:
DOI: 10.2210/pdb4ksr/pdb
PDB entry 4ksr coloured by chain, front view.
PDB entry 4ksr coloured by chain, side view.
PDB entry 4ksr coloured by chain, top view.
Source organisms:
Primary publication:
Hexamers of the type II secretion ATPase GspE from Vibrio cholerae with increased ATPase activity.
Lu C, Turley S, Marionni ST, Park YJ, Lee KK, Patrick M, Shah R, Sandkvist M, Bush MF, Hol WG
Structure 21 1707-17 (2013)
PMID: 23954505

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system ATPase E; Type VI secretion system tube protein Hcp Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 583 amino acids
Theoretical weight: 64.29 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37093 (Residues: 100-503; Coverage: 80%)
  • Canonical: A0A0H2ZJ24 (Residues: 1-162; Coverage: 100%)
Gene names: PA14_01030, VC_2732, epsE
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Unit cell:
a: 112.467Å b: 132.911Å c: 142.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.384 0.384 0.376
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
6 more

8 mentions without citation

Super-Resolution Imaging of Protein Secretion Systems and the Cell Surface of Gram-Negative Bacteria.
Gunasinghe et al. (2017)
7 more