› 4kt3

X-ray diffraction
1.44Å resolution

Structure of a type VI secretion system effector-immunity complex from Pseudomonas protegens

Released:
DOI: 10.2210/pdb4kt3/pdb
PDB entry 4kt3 coloured by chain, front view.
PDB entry 4kt3 coloured by chain, side view.
PDB entry 4kt3 coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Primary publication:
Identification, structure, and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair.
Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD
J Biol Chem 288 26616-24 (2013)
PMID: 23878199

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175614 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 18.96 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4KC90 (Residues: 1-177; Coverage: 100%)
Gene name: PFL_3037
Sequence domains: Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
Structure domains: Lysozyme
Putative lipoprotein Chain: B
Molecule details ›
Chain: B
Length: 137 amino acids
Theoretical weight: 15.41 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4KC91 (Residues: 24-156; Coverage: 100%)
Gene name: PFL_3036
Sequence domains: T6SS, Immunity protein Tgi2PP
Structure domains: type vi secretion system effector-immunity co pseudomonas protegens

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 40.112Å b: 81.515Å c: 86.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.175
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Type VI secretion system effectors: poisons with a purpose.
Russell et al. (2014)
16 more

3 mentions without citation

Molecular weaponry: diverse effectors delivered by the Type VI secretion system.
Alcoforado Diniz et al. (2015)
2 more