› 4kul

X-ray diffraction
2.62Å resolution

Crystal structure of N-terminal acetylated yeast Sir3 BAH domain V83P mutant

Released:
DOI: 10.2210/pdb4kul/pdb
PDB entry 4kul coloured by chain, front view.
PDB entry 4kul coloured by chain, side view.
PDB entry 4kul coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Nα-acetylated Sir3 stabilizes the conformation of a nucleosome-binding loop in the BAH domain.
Yang D, Fang Q, Wang M, Ren R, Wang H, He M, Sun Y, Yang N, Xu RM
Nat Struct Mol Biol 20 1116-8 (2013)
PMID: 23934152

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein SIR3 Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 26.77 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06701 (Residues: 2-219; Coverage: 22%)
Gene names: CMT1, L9753.10, MAR2, SIR3, STE8, YLR442C
Sequence domains: BAH domain
Structure domains: SH3 type barrels.

Ligands and Environments

No bound ligands
1 modified residue:
Ligand AYA 1 x AYA

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 94.227Å b: 43.453Å c: 53.636Å
α: 90° β: 97.43° γ: 90°
R-values:
R R work R free
0.221 0.217 0.277
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Nucleosome structure and function.
McGinty et al. (2015)
14 more