› 4kuo

X-ray diffraction
2Å resolution

A superfast recovering full-length LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae (Photoexcited state)

Released:
DOI: 10.2210/pdb4kuo/pdb
PDB entry 4kuo coloured by chain, front view.
PDB entry 4kuo coloured by chain, side view.
PDB entry 4kuo coloured by chain, top view.
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Primary publication:
Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae.
Endres S, Granzin J, Circolone F, Stadler A, Krauss U, Drepper T, Svensson V, Knieps-Grünhagen E, Wirtz A, Cousin A, Tielen P, Willbold D, Jaeger KE, Batra-Safferling R
BMC Microbiol 15 30 (2015)
PMID: 25887755

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative blue-light photoreceptor Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.86 KDa
Source organism: Dinoroseobacter shibae DFL 12 = DSM 16493
Expression system: Escherichia coli
UniProt:
  • Canonical: A8LP63 (Residues: 2-139; Coverage: 99%)
Gene name: Dshi_2006
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand RBF 1 x RBF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 90.78Å b: 30.936Å c: 49.538Å
α: 90° β: 113.09° γ: 90°
R-values:
R R work R free
0.164 0.163 0.182
Expression system: Escherichia coli

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Citations

1 review citation

Applications of hydrogen deuterium exchange (HDX) for the characterization of conformational dynamics in light-activated photoreceptors.
Lindner et al. (2015)
10 other articles

1 mention without citation

Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae.
Endres et al. (2015)