› 4kvz

X-ray diffraction
1.75Å resolution

Crystal structure of the plantazolicin methyltransferase BamL in complex with SAH

Released:
DOI: 10.2210/pdb4kvz/pdb
PDB entry 4kvz coloured by chain, front view.
PDB entry 4kvz coloured by chain, side view.
PDB entry 4kvz coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Structural and functional insight into an unexpectedly selective N-methyltransferase involved in plantazolicin biosynthesis.
Lee J, Hao Y, Blair PM, Melby JO, Agarwal V, Burkhart BJ, Nair SK, Mitchell DA
Proc Natl Acad Sci U S A 110 12954-9 (2013)
PMID: 23878226

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 30.88 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: D3VMM1 (Residues: 1-270; Coverage: 100%)
Gene name: ptnL
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 78.14Å b: 80.08Å c: 88.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.247
Expression system: Escherichia coli

Quick links

Citations

5 review citations

YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function.
Burkhart et al. (2017)
4 more

4 mentions without citation

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
3 more