› 4kx6

X-ray diffraction
2.95Å resolution

Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase

Released:
DOI: 10.2210/pdb4kx6/pdb
PDB entry 4kx6 coloured by chain, front view.
PDB entry 4kx6 coloured by chain, side view.
PDB entry 4kx6 coloured by chain, top view.
Source organisms:
Primary publication:
Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase.
Singh PK, Sarwar M, Maklashina E, Kotlyar V, Rajagukguk S, Tomasiak TM, Cecchini G, Iverson TM
J Biol Chem 288 24293-301 (2013)
PMID: 23836905

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-132437 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Fumarate reductase flavoprotein subunit Chains: A, M
Molecule details ›
Chains: A, M
Length: 577 amino acids
Theoretical weight: 63.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00363 (Residues: 1-577; Coverage: 96%)
Gene names: JW4115, b4154, frdA
Sequence domains:
Structure domains:
Fumarate reductase (Anaerobic), Fe-S subunit Chains: B, N
Molecule details ›
Chains: B, N
Length: 243 amino acids
Theoretical weight: 27.02 KDa
Source organism: Escherichia coli BW2952
Expression system: Escherichia coli
Structure domains:
Fumarate reductase subunit C Chains: C, O
Molecule details ›
Chains: C, O
Length: 130 amino acids
Theoretical weight: 14.88 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit
Fumarate reductase subunit D Chains: D, P
Molecule details ›
Chains: D, P
Length: 119 amino acids
Theoretical weight: 13.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8Q3 (Residues: 1-119; Coverage: 100%)
Gene names: JW4112, b4151, frdD
Sequence domains: Fumarate reductase subunit D
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
4 bound ligands:
Ligand FES 2 x FES
Ligand F3S 2 x F3S
Ligand SF4 2 x SF4
Ligand MQ7 2 x MQ7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 97.093Å b: 138.62Å c: 273.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.24 0.28
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Infrared spectroscopic markers of quinones in proteins from the respiratory chain.
Hellwig P. (2015)
4 other articles

1 mention without citation

Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase.
Singh et al. (2013)