› 4kxt

X-ray diffraction
2.29Å resolution

Structure of human ARGONAUTE1 in complex with guide RNA

Released:
DOI: 10.2210/pdb4kxt/pdb
PDB entry 4kxt coloured by chain, front view.
PDB entry 4kxt coloured by chain, side view.
PDB entry 4kxt coloured by chain, top view.
Source organisms:
Primary publication:
Eukaryote-specific insertion elements control human ARGONAUTE slicer activity.
Nakanishi K, Ascano M, Gogakos T, Ishibe-Murakami S, Serganov AA, Briskin D, Morozov P, Tuschl T, Patel DJ
Cell Rep 3 1893-900 (2013)
PMID: 23809764

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193903 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-1 Chain: A
Molecule details ›
Chain: A
Length: 862 amino acids
Theoretical weight: 97.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UL18 (Residues: 1-857; Coverage: 100%)
Gene names: AGO1, EIF2C1
Sequence domains:
Structure domains:
RNA Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.86 KDa
Source organism: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 69.879Å b: 100.249Å c: 136.701Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.228
Expression system: Spodoptera frugiperda

Quick links

Citations

20 review citations

The evolutionary journey of Argonaute proteins.
Swarts et al. (2014)
19 more

11 mentions without citation

Anatomy of RISC: how do small RNAs and chaperones activate Argonaute proteins?
Nakanishi K. (2016)
10 more