› 4kyb

X-ray diffraction
2.91Å resolution

Crystal Structure of de novo designed serine hydrolase OSH55.14_E3, Northeast Structural Genomics Consortium Target OR342

Released:
DOI: 10.2210/pdb4kyb/pdb
PDB entry 4kyb coloured by chain, front view.
PDB entry 4kyb coloured by chain, side view.
PDB entry 4kyb coloured by chain, top view.
Source organism: synthetic construct
Entry authors: Kuzin A, Lew S, Rajagopalan S, Seetharaman J, Mao L, Xiao R, Lee D, Raja S, Everett JK, Acton TB, Baker D, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Designed Protein OR342 Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 17.74 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Leucine Aminopeptidase, subunit E, domain 1

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P212121
Unit cell:
a: 30.054Å b: 76.401Å c: 132.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.201 0.301
Expression system: Escherichia coli BL21(DE3)

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