› 4kyc

X-ray diffraction
1.95Å resolution

Structure of the C-terminal domain of the Menangle virus phosphoprotein, fused to MBP.

Released:
DOI: 10.2210/pdb4kyc/pdb
PDB entry 4kyc coloured by chain, front view.
PDB entry 4kyc coloured by chain, side view.
PDB entry 4kyc coloured by chain, top view.
Source organisms:
Primary publication:
Protein domain definition should allow for conditional disorder.
Yegambaram K, Bulloch EM, Kingston RL
Protein Sci 22 1502-18 (2013)
PMID: 23963781

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Phosphoprotein Chain: A
Molecule details ›
Chain: A
Length: 420 amino acids
Theoretical weight: 46.12 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q91MK1 (Residues: 339-388; Coverage: 13%)
Gene names: JW3994, V/P, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand EDO 1 x EDO
Ligand BO3 1 x BO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 77.869Å b: 77.869Å c: 197.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Intrinsically disordered proteins and intrinsically disordered protein regions.
Oldfield et al. (2014)
4 more

4 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
3 more