› 4kyt

X-ray diffraction
2.83Å resolution

The structure of superinhibitory phospholamban bound to the calcium pump SERCA1a

Released:
DOI: 10.2210/pdb4kyt/pdb
PDB entry 4kyt coloured by chain, front view.
PDB entry 4kyt coloured by chain, side view.
PDB entry 4kyt coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis for phospholamban inhibition of the calcium pump in sarcoplasmic reticulum.
Akin BL, Hurley TD, Chen Z, Jones LR
J Biol Chem 288 30181-30191 (2013)
PMID: 23996003

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
hetero trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-137503 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: A
Molecule details ›
Chain: A
Length: 994 amino acids
Theoretical weight: 109.6 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 1-993; Coverage: 99%)
Gene name: ATP2A1
Sequence domains:
Structure domains:
Cardiac phospholamban Chains: B, C
Molecule details ›
Chains: B, C
Length: 52 amino acids
Theoretical weight: 5.95 KDa
Source organism: Canis lupus familiaris
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P61012 (Residues: 1-52; Coverage: 100%)
Gene name: PLN
Sequence domains: Phospholamban

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 61.591Å b: 93.094Å c: 316.375Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.284
Expression system: Spodoptera frugiperda

Quick links

Citations

10 review citations

Molecular dynamics simulations of biological membranes and membrane proteins using enhanced conformational sampling algorithms.
Mori et al. (2016)
9 more

16 mentions without citation

Linking Biochemical and Structural States of SERCA: Achievements, Challenges, and New Opportunities.
Aguayo-Ortiz et al. (2020)
15 more