› 4kyz

X-ray diffraction
2.49Å resolution

Three-dimensional structure of triclinic form of de novo design insertion domain, Northeast Structural Genomics Consortium (NESG) Target OR327

Released:
DOI: 10.2210/pdb4kyz/pdb
PDB entry 4kyz coloured by chain, front view.
PDB entry 4kyz coloured by chain, side view.
PDB entry 4kyz coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Precise assembly of complex beta sheet topologies from de novo designed building blocks.
King IC, Gleixner J, Doyle L, Kuzin A, Hunt JF, Xiao R, Montelione GT, Stoddard BL, DiMaio F, Baker D
Elife 4 (2015)
PMID: 26650357

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Designed protein OR327 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 172 amino acids
Theoretical weight: 19.84 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Ribosomal protein S10 domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 43.819Å b: 55.402Å c: 78.258Å
α: 82.03° β: 80.34° γ: 90.03°
R-values:
R R work R free
0.195 0.191 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Biomolecular Assemblies: Moving from Observation to Predictive Design.
Wilson et al. (2018)
2 more

1 mention without citation

Precise assembly of complex beta sheet topologies from de novo designed building blocks.
King et al. (2015)