› 4l07

X-ray diffraction
1.75Å resolution

Crystal structure of the maleamate amidase Ami from Pseudomonas putida S16

Released:
DOI: 10.2210/pdb4l07/pdb
Model geometry
Fit model/data
PDB entry 4l07 coloured by chain, front view.
PDB entry 4l07 coloured by chain, side view.
PDB entry 4l07 coloured by chain, top view.
Source organism: Pseudomonas putida S16
Primary publication:
Structural insights into the specific recognition of N-heterocycle biodenitrogenation-derived substrates by microbial amide hydrolases.
Wu G, Chen D, Tang H, Ren Y, Chen Q, Lv Y, Zhang Z, Zhao YL, Yao Y, Xu P
Mol Microbiol 91 1009-21 (2014)
PMID: 24397579

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maleamate amidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 22.17 KDa
Source organism: Pseudomonas putida S16
Expression system: Escherichia coli
UniProt:
  • Canonical: F8G0M0 (Residues: 1-208; Coverage: 100%)
Sequence domains: Isochorismatase domain
Structure domains: Isochorismatase-like

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
Spacegroup: P6422
Unit cell:
a: 133.489Å b: 133.489Å c: 88.844Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.198 0.221
Expression system: Escherichia coli

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Citations

1 review citation

Physiology of a Hybrid Pathway for Nicotine Catabolism in Bacteria.
Huang et al. (2020)
4 other articles