› 4l0g

X-ray diffraction
2Å resolution

Crystal Structure of a GH48 cellobiohydrolase from Caldicellulosiruptor bescii

Released:
DOI: 10.2210/pdb4l0g/pdb
Model geometry
Fit model/data
PDB entry 4l0g coloured by chain, front view.
PDB entry 4l0g coloured by chain, side view.
PDB entry 4l0g coloured by chain, top view.
Source organism: Caldicellulosiruptor bescii DSM 6725
Entry authors: Jiao A, Bai A, Yan F, Geng W

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110865 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 74.62 KDa
Source organism: Caldicellulosiruptor bescii DSM 6725
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9MKU7 (Residues: 1122-1759; Coverage: 36%)
Gene name: Athe_1867
Sequence domains: Glycosyl hydrolase family 48
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand EDO 6 x EDO
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 116.313Å b: 57.225Å c: 105.897Å
α: 90° β: 103.38° γ: 90°
R-values:
R R work R free
0.196 0.195 0.219
Expression system: Escherichia coli BL21(DE3)

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