› 4l1f

X-ray diffraction
1.79Å resolution

Electron transferring flavoprotein of Acidaminococcus fermentans: Towards a mechanism of flavin-based electron bifurcation

Released:
DOI: 10.2210/pdb4l1f/pdb
PDB entry 4l1f coloured by chain, front view.
PDB entry 4l1f coloured by chain, side view.
PDB entry 4l1f coloured by chain, top view.
Source organism: Acidaminococcus fermentans DSM 20731
Primary publication:
Studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) of Acidaminococcus fermentans.
Chowdhury NP, Mowafy AM, Demmer JK, Upadhyay V, Koelzer S, Jayamani E, Kahnt J, Hornung M, Demmer U, Ermler U, Buckel W
J Biol Chem 289 5145-57 (2014)
PMID: 24379410

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-CoA dehydrogenase domain protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 41.88 KDa
Source organism: Acidaminococcus fermentans DSM 20731
UniProt:
  • Canonical: D2RL84 (Residues: 1-383; Coverage: 100%)
Gene name: Acfer_1477
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand FAD 2 x FAD
Ligand COS 2 x COS
Ligand NA 3 x NA
Ligand PDO 1 x PDO
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 109.1Å b: 141.4Å c: 64.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.153 0.183

Quick links

Citations

12 review citations

Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria.
Schuchmann et al. (2014)
11 more

2 mentions without citation

Studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) of Acidaminococcus fermentans.
Chowdhury et al. (2014)
1 more