› 4l2a

X-ray diffraction
2.06Å resolution

X-ray structure of the C57R mutant of the iron superoxide dismutase from Pseudoalteromonas haloplanktis (crystal form II)

Released:
DOI: 10.2210/pdb4l2a/pdb
PDB entry 4l2a coloured by chain, front view.
PDB entry 4l2a coloured by chain, side view.
PDB entry 4l2a coloured by chain, top view.
Source organism: Pseudoalteromonas haloplanktis
Primary publication:
Structural and denaturation studies of two mutants of a cold adapted superoxide dismutase point to the importance of electrostatic interactions in protein stability.
Merlino A, Russo Krauss I, Castellano I, Ruocco MR, Capasso A, De Vendittis E, Rossi B, Sica F
Biochim Biophys Acta 1844 632-40 (2014)
PMID: 24440460

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Fe] Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.32 KDa
Source organism: Pseudoalteromonas haloplanktis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84612 (Residues: 1-192; Coverage: 100%)
Gene names: PSHAa1215, sodB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 45.622Å b: 103.736Å c: 50.244Å
α: 90° β: 108.29° γ: 90°
R-values:
R R work R free
0.19 0.19 0.24
Expression system: Escherichia coli

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Citations

3 citation in other articles

α-Chymotrypsin regulates free fatty acids and UCHL-1 to ameliorate N-methyl nitrosourea induced mammary gland carcinoma in albino wistar rats.
Rani et al. (2016)
2 more