4l2n

X-ray diffraction
1.74Å resolution

Understanding Extradiol Dioxygenase Mechanism in NAD+ Biosynthesis by Viewing Catalytic Intermediates - ligand-free structure

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
3-hydroxyanthranilate + O(2) = 2-amino-3-carboxymuconate semialdehyde
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-172947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyanthranilate 3,4-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.06 KDa
Source organism: Cupriavidus metallidurans CH34
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q1LCS4 (Residues: 1-174; Coverage: 100%)
Gene names: Rmet_5193, nbaC
Sequence domains: 3-hydroxyanthranilic acid dioxygenase
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6522
Unit cell:
a: 58.34Å b: 58.34Å c: 231.426Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.211 0.254
Expression system: Escherichia coli BL21