› 4l3k

X-ray diffraction
1.88Å resolution

Crystal structure of Sporosarcina pasteurii UreE bound to Ni2+ and Zn2+

Released:
DOI: 10.2210/pdb4l3k/pdb
PDB entry 4l3k coloured by chain, front view.
PDB entry 4l3k coloured by chain, side view.
PDB entry 4l3k coloured by chain, top view.
Source organism: Sporosarcina pasteurii
Primary publication:
Selectivity of Ni(II) and Zn(II) binding to Sporosarcina pasteurii UreE, a metallochaperone in the urease assembly: a calorimetric and crystallographic study.
Zambelli B, Banaszak K, Merloni A, Kiliszek A, Rypniewski W, Ciurli S
J Biol Inorg Chem 18 1005-17 (2013)
PMID: 24126709

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urease accessory protein UreE Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 17.43 KDa
Source organism: Sporosarcina pasteurii
Expression system: Escherichia coli
UniProt:
  • Canonical: P50049 (Residues: 1-147; Coverage: 100%)
Gene name: ureE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P63
Unit cell:
a: 91.329Å b: 91.329Å c: 82.792Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.207
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
3 more

2 mentions without citation

Evolution of Macromolecular Docking Techniques: The Case Study of Nickel and Iron Metabolism in Pathogenic Bacteria.
Musiani et al. (2015)
1 more