› 4l51

X-ray diffraction
1.9Å resolution

Crystal structures of the LsrR proteins complexed with phospho-AI-2 and its two different analogs reveal distinct mechanisms for ligand recognition

Released:
DOI: 10.2210/pdb4l51/pdb
PDB entry 4l51 coloured by chain, front view.
PDB entry 4l51 coloured by chain, side view.
PDB entry 4l51 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structures of the LsrR proteins complexed with phospho-AI-2 and two signal-interrupting analogues reveal distinct mechanisms for ligand recognition.
Ha JH, Eo Y, Grishaev A, Guo M, Smith JA, Sintim HO, Kim EH, Cheong HK, Bentley WE, Ryu KS
J Am Chem Soc 135 15526-35 (2013)
PMID: 24047255

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator LsrR Chains: A, B
Molecule details ›
Chains: A, B
Length: 266 amino acids
Theoretical weight: 27.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76141 (Residues: 53-317; Coverage: 84%)
Gene names: JW1505, b1512, lsrR, ydeW
Sequence domains: Putative sugar-binding domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand HSX 2 x HSX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P65
Unit cell:
a: 116.613Å b: 116.613Å c: 79.744Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.204
Expression system: Escherichia coli

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Citations

1 review citation

Antimicrobial Resistance and Recent Alternatives to Antibiotics for the Control of Bacterial Pathogens with an Emphasis on Foodborne Pathogens.
Helmy et al. (2023)
11 other articles

2 mentions without citation

Mechanisms of Inhibition of Quorum Sensing as an Alternative for the Control of E. coli and Salmonella.
Escobar-Muciño et al. (2022)
1 more