› 4l5f

X-ray diffraction
2.45Å resolution

Crystal Structure of DENV1-E106 Fab bound to DENV-1 Envelope protein DIII

Released:
DOI: 10.2210/pdb4l5f/pdb
PDB entry 4l5f coloured by chain, front view.
PDB entry 4l5f coloured by chain, side view.
PDB entry 4l5f coloured by chain, top view.
Source organisms:
Entry authors: Edeling MA, Nelson CA, Fremont DH, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212566 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heavy chain of E106 antibody (VH and CH1 of IgG2c) Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.54 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Envelope protein Chain: E
Molecule details ›
Chain: E
Length: 111 amino acids
Theoretical weight: 11.99 KDa
Source organism: dengue virus type 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8BE40 (Residues: 293-399; Coverage: 22%)
Gene name: E
Sequence domains: Flavivirus glycoprotein, immunoglobulin-like domain
Structure domains: Immunoglobulin-like
Light chain of E106 antibody (kappa) Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.56 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 82.661Å b: 91.823Å c: 92.599Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.239
Expression system: Escherichia coli BL21(DE3)

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