› 4l5g

X-ray diffraction
2.39Å resolution

Crystal structure of Thermus thermophilus CarD

Released:
DOI: 10.2210/pdb4l5g/pdb
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PDB entry 4l5g coloured by chain, front view.
PDB entry 4l5g coloured by chain, side view.
PDB entry 4l5g coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structure and function of CarD, an essential mycobacterial transcription factor.
Srivastava DB, Leon K, Osmundson J, Garner AL, Weiss LA, Westblade LF, Glickman MS, Landick R, Darst SA, Stallings CL, Campbell EA
Proc Natl Acad Sci U S A 110 12619-24 (2013)
PMID: 23858468

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CarD-like/TRCF RNAP-interacting domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 18.54 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLX5 (Residues: 1-164; Coverage: 100%)
Gene name: TTHA0168
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 75.128Å b: 136.085Å c: 104.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.23
Expression system: Escherichia coli

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Citations

13 review citations

Local and global regulation of transcription initiation in bacteria.
Browning et al. (2016)
12 more

6 mentions without citation

Structure and function of CarD, an essential mycobacterial transcription factor.
Srivastava et al. (2013)
5 more

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