› 4l74

X-ray diffraction
1.84Å resolution

Ca2+-bound MthK RCK domain at 1.9 Angstrom with single ligand

Released:
DOI: 10.2210/pdb4l74/pdb
PDB entry 4l74 coloured by chain, front view.
PDB entry 4l74 coloured by chain, side view.
PDB entry 4l74 coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Primary publication:
Structural basis of allosteric interactions among Ca2+-binding sites in a K+ channel RCK domain.
Smith FJ, Pau VP, Cingolani G, Rothberg BS
Nat Commun 4 2621 (2013)
PMID: 24126388

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 26.89 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli
UniProt:
  • Canonical: O27564 (Residues: 107-336; Coverage: 69%)
Gene names: MTH_1520, mthK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 58.233Å b: 38.424Å c: 96.821Å
α: 90° β: 94.32° γ: 90°
R-values:
R R work R free
0.213 0.211 0.253
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure of potassium channels.
Kuang et al. (2015)
2 more

1 mention without citation

3dDNA: A Computational Method of Building DNA 3D Structures.
Zhang et al. (2022)