4l79

X-ray diffraction
2.3Å resolution

Crystal Structure of nucleotide-free Myosin 1b residues 1-728 with bound Calmodulin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-143940 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Unconventional myosin-Ib Chain: A
Molecule details ›
Chain: A
Length: 744 amino acids
Theoretical weight: 86 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q05096 (Residues: 1-728; Coverage: 64%)
Gene names: Myo1a, Myo1b, Myr1
Sequence domains:
Calmodulin-1 Chain: B
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 114.184Å b: 45.09Å c: 115.547Å
α: 90° β: 117.18° γ: 90°
R-values:
R R work R free
0.192 0.189 0.249
Expression system: Spodoptera frugiperda