› 4l8h

X-ray diffraction
2.4Å resolution

Bacteriophage Qbeta coat protein in complex with RNA operator hairpin

Released:
DOI: 10.2210/pdb4l8h/pdb
PDB entry 4l8h coloured by chain, front view.
PDB entry 4l8h coloured by chain, side view.
PDB entry 4l8h coloured by chain, top view.
Source organism: Qubevirus durum
Primary publication:
Crystal structure of the bacteriophage Qβ coat protein in complex with the RNA operator of the replicase gene.
Rumnieks J, Tars K
J Mol Biol 426 1039-49 (2014)
PMID: 24035813

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137174 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Capsid protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia virus Qbeta
Expression system: Escherichia coli
UniProt:
  • Canonical: P03615 (Residues: 2-133; Coverage: 99%)
Sequence domains: Levivirus coat protein
Structure domains: MS2 Viral Coat Protein
RNA operator hairpin Chain: R
Molecule details ›
Chain: R
Length: 20 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Qubevirus durum
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P6522
Unit cell:
a: 75.84Å b: 75.84Å c: 303.49Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.253 0.288
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Virus Isoelectric Point Estimation: Theories and Methods.
Heffron et al. (2021)
1 more

5 mentions without citation

Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ.
Gorzelnik et al. (2016)
4 more