› 4l9o

X-ray diffraction
1.6Å resolution

Crystal Structure of the Sec13-Sec16 blade-inserted complex from Pichia pastoris

Released:
DOI: 10.2210/pdb4l9o/pdb
PDB entry 4l9o coloured by chain, front view.
PDB entry 4l9o coloured by chain, side view.
PDB entry 4l9o coloured by chain, top view.
Source organisms:
Primary publication:
Sec16 influences transitional ER sites by regulating rather than organizing COPII.
Bharucha N, Liu Y, Papanikou E, McMahon C, Esaki M, Jeffrey PD, Hughson FM, Glick BS
Mol Biol Cell 24 3406-19 (2013)
PMID: 24006484

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein transport protein SEC13; Protein transport protein sec16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 38.49 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q45TY0 (Residues: 1030-1076; Coverage: 2%)
  • Canonical: P53024 (Residues: 2-289; Coverage: 100%)
Gene names: PAS_chr1-3_0057, SEC13, SEC16
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand CL 2 x CL
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 70.509Å b: 49.32Å c: 90.904Å
α: 90° β: 111.7° γ: 90°
R-values:
R R work R free
0.174 0.173 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

The pathway of collagen secretion.
Malhotra et al. (2015)
14 more

2 mentions without citation

Sec16 influences transitional ER sites by regulating rather than organizing COPII.
Bharucha et al. (2013)
1 more