› 4l9t

X-ray diffraction
1.79Å resolution

Crystal structure of MepR F27L mutant from multidrug resistant S. aureus clinical isolate

Released:
DOI: 10.2210/pdb4l9t/pdb
PDB entry 4l9t coloured by chain, front view.
PDB entry 4l9t coloured by chain, side view.
PDB entry 4l9t coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
The molecular mechanisms of allosteric mutations impairing MepR repressor function in multidrug-resistant strains of Staphylococcus aureus.
Birukou I, Tonthat NK, Seo SM, Schindler BD, Kaatz GW, Brennan RG
mBio 4 e00528-13 (2013)
PMID: 23982071

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH marR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.99 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5Y812 (Residues: 1-139; Coverage: 100%)
Gene names: BN1321_80052, E1948_01710, E1948_06500, EP54_02880, EQ90_08705, FAF17_12965, G6W63_10695, G6Y24_06545, GO793_15615, GO814_10745, GO941_13505, GO942_09445, GQX37_11080, GZ156_06425, GZ163_02140, H2639_11915, M1K003_0421, NCTC10702_00659, NCTC13131_05788, NCTC5664_00388, NCTC7878_00347, NCTC7972_01611, SAMEA1531744_01160, SAMEA2078260_01697, SAMEA2078588_01240, SAMEA2080344_01299, SAMEA2081063_00451, SAMEA4008575_01717, SAMEA70146418_01252, SAMEA70153168_00678, mepR
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 32.441Å b: 113.122Å c: 49.936Å
α: 90° β: 98.18° γ: 90°
R-values:
R R work R free
0.172 0.17 0.203
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Antibiotics: Pharmacokinetics, toxicity, resistance and multidrug efflux pumps.
Yılmaz et al. (2017)
1 more