› 4lb8

X-ray diffraction
2.49Å resolution

Crystal structure of a DUF4848 family protein (BT3222) from Bacteroides thetaiotaomicron VPI-5482 at 2.49 A resolution

Released:
DOI: 10.2210/pdb4lb8/pdb
PDB entry 4lb8 coloured by chain, front view.
PDB entry 4lb8 coloured by chain, side view.
PDB entry 4lb8 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF4848 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.18 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A2T3 (Residues: 22-310; Coverage: 100%)
Gene name: BT_3222
Sequence domains: Domain of unknown function (DUF4848)
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:
Ligand CL 5 x CL
Ligand PEG 2 x PEG
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2221
Unit cell:
a: 64.595Å b: 140.829Å c: 89.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.228
Expression system: Escherichia coli

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