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› 4lcg

X-ray diffraction
1.57Å resolution

Crystal structure of the Pseudomonas aeruginosa LPXC/LPC-050 complex

Released:
DOI: 10.2210/pdb4lcg/pdb
Model geometry
Fit model/data
PDB entry 4lcg coloured by chain, front view.
PDB entry 4lcg coloured by chain, side view.
PDB entry 4lcg coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Synthesis, structure, and antibiotic activity of aryl-substituted LpxC inhibitors.
Liang X, Lee CJ, Zhao J, Toone EJ, Zhou P
J Med Chem 56 6954-6966 (2013)
PMID: 23914798

Function and Biology Details

Reaction catalysed: 
a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine + H2O =a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + acetate.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-3-O-acyl-N-acetylglucosamine deacetylase Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 33.15 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47205 (Residues: 1-299; Coverage: 99%)
Gene names: PA4406, envA, lpxC
Sequence domains: UDP-3-O-acyl N-acetylglycosamine deacetylase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand 1WM 1 x 1WM
Ligand NO3 16 x NO3
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 53.53Å b: 73.62Å c: 88.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.206
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Biosynthesis and export of bacterial lipopolysaccharides.
Whitfield et al. (2014)
5 more

1 mention without citation

Synthesis, structure, and antibiotic activity of aryl-substituted LpxC inhibitors.
Liang et al. (2013)