› 4ldy

X-ray diffraction
2.3Å resolution

Crystal structure of the DNA binding domain of the G245A mutant of arabidopsis thaliana auxin reponse factor 1

Released:
DOI: 10.2210/pdb4ldy/pdb
PDB entry 4ldy coloured by chain, front view.
PDB entry 4ldy coloured by chain, side view.
PDB entry 4ldy coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for DNA binding specificity by the auxin-dependent ARF transcription factors.
Boer DR, Freire-Rios A, van den Berg WA, Saaki T, Manfield IW, Kepinski S, López-Vidrieo I, Franco-Zorrilla JM, de Vries SC, Solano R, Weijers D, Coll M
Cell 156 577-89 (2014)
PMID: 24485461

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Auxin response factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 41.09 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8L7G0 (Residues: 1-355; Coverage: 53%)
Gene names: ARF1, At1g59750, F23H11.7
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P21
Unit cell:
a: 73.1Å b: 84.24Å c: 78.96Å
α: 90° β: 114.76° γ: 90°
R-values:
R R work R free
0.213 0.21 0.259
Expression system: Escherichia coli

Quick links

Citations

56 review citations

The yin-yang of hormones: cytokinin and auxin interactions in plant development.
Schaller et al. (2015)
55 more