› 4lg2

X-ray diffraction
2.7Å resolution

Crystal structure of Reston Ebola virus VP35 RNA binding domain bound to 12-bp dsRNA

Released:
DOI: 10.2210/pdb4lg2/pdb
PDB entry 4lg2 coloured by chain, front view.
PDB entry 4lg2 coloured by chain, side view.
PDB entry 4lg2 coloured by chain, top view.
Source organism: Reston ebolavirus - Reston (1989)
Primary publication:
Ebolavirus VP35 coats the backbone of double-stranded RNA for interferon antagonism.
Bale S, Julien JP, Bornholdt ZA, Krois AS, Wilson IA, Saphire EO
J Virol 87 10385-8 (2013)
PMID: 23824825

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185012 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Polymerase cofactor VP35 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 146 amino acids
Theoretical weight: 16.56 KDa
Source organism: Reston ebolavirus - Reston (1989)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JPY0 (Residues: 205-329; Coverage: 38%)
Gene names: REBOVgp2, VP35
Sequence domains: Filoviridae VP35
Structure domains:
dsRNA Chains: E, F, I, J
Molecule details ›
Chains: E, F, I, J
Length: 12 nucleotides
Theoretical weight: 3.81 KDa
Source organism: Reston ebolavirus - Reston (1989)
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 55.09Å b: 72.871Å c: 175.551Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.262
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Strategies of highly pathogenic RNA viruses to block dsRNA detection by RIG-I-like receptors: hide, mask, hit.
Zinzula et al. (2013)
9 more

5 mentions without citation

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
4 more