› 4lj0

X-ray diffraction
2.15Å resolution

Nab2 Zn fingers complexed with polyadenosine

Released:
DOI: 10.2210/pdb4lj0/pdb
PDB entry 4lj0 coloured by chain, front view.
PDB entry 4lj0 coloured by chain, side view.
PDB entry 4lj0 coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
Structural basis for the molecular recognition of polyadenosine RNA by Nab2 Zn fingers.
Kuhlmann SI, Valkov E, Stewart M
Nucleic Acids Res 42 672-80 (2014)
PMID: 24071581

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124072 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nuclear polyadenylated RNA-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 66 amino acids
Theoretical weight: 7.52 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SCL7 (Residues: 401-466; Coverage: 13%)
Gene name: CTHT_0057680
Sequence domains: RNA-binding, Nab2-type zinc finger
Structure domains: CCCH zinc finger
polyadenosine RNA Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 8 nucleotides
Theoretical weight: 2.59 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand ACT 2 x ACT
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3121
Unit cell:
a: 90.929Å b: 90.929Å c: 54.081Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.207
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Poly(A) RNA-binding proteins and polyadenosine RNA: new members and novel functions.
Wigington et al. (2014)
1 more

2 mentions without citation

The structure of the ubiquitin-like modifier FAT10 reveals an alternative targeting mechanism for proteasomal degradation.
Aichem et al. (2018)
1 more