› 4ljy

X-ray diffraction
1.95Å resolution

Crystal structure of RNA splicing effector Prp5 in complex with ADP

Released:
DOI: 10.2210/pdb4ljy/pdb
PDB entry 4ljy coloured by chain, front view.
PDB entry 4ljy coloured by chain, side view.
PDB entry 4ljy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of Prp5p reveals interdomain interactions that impact spliceosome assembly.
Zhang ZM, Yang F, Zhang J, Tang Q, Li J, Gu J, Zhou J, Xu YZ
Cell Rep 5 1269-78 (2013)
PMID: 24290758

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-processing ATP-dependent RNA helicase PRP5 Chain: A
Molecule details ›
Chain: A
Length: 493 amino acids
Theoretical weight: 55.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P21372 (Residues: 206-698; Coverage: 58%)
Gene names: PRP5, RNA5, YBR1603, YBR237W
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand MRD 2 x MRD
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 86.598Å b: 86.598Å c: 125.316Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.226
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Functions and regulation of the Brr2 RNA helicase during splicing.
Absmeier et al. (2016)
5 more

3 mentions without citation

SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.
Carrocci et al. (2017)
2 more