› 4lkk

X-ray diffraction
2.49Å resolution

The structure of hemagglutinin L226Q mutant (H3 numbering) from a avian-origin H7N9 influenza virus (A/Anhui/1/2013) in complex with human receptor analog 6'SLNLN

Released:
DOI: 10.2210/pdb4lkk/pdb
PDB entry 4lkk coloured by chain, front view.
PDB entry 4lkk coloured by chain, side view.
PDB entry 4lkk coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Structures and receptor binding of hemagglutinins from human-infecting H7N9 influenza viruses.
Shi Y, Zhang W, Wang F, Qi J, Wu Y, Song H, Gao F, Bi Y, Zhang Y, Fan Z, Qin C, Sun H, Liu J, Haywood J, Liu W, Gong W, Wang D, Shu Y, Wang Y, Yan J, Gao GF
Science 342 243-7 (2013)
PMID: 24009358

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197612 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 314 amino acids
Theoretical weight: 34.21 KDa
Source organism: Influenza A virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: V5IRV5 (Residues: 1-314; Coverage: 100%)
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 19.43 KDa
Source organism: Influenza A virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: V5IRV2 (Residues: 1-168; Coverage: 100%)
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R32
Unit cell:
a: 117.143Å b: 117.143Å c: 297.664Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.236 0.271
Expression system: Spodoptera frugiperda

Quick links

Citations

33 review citations

Role of receptor binding specificity in influenza A virus transmission and pathogenesis.
de Graaf et al. (2014)
32 more

2 mentions without citation

New insights into influenza A specificity: an evolution of paradigms.
Ji et al. (2017)
1 more