4llg

X-ray diffraction
3.79Å resolution

Crystal Structure Analysis of the E.coli holoenzyme/Gp2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-132729 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 239 amino acids
Theoretical weight: 26.46 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
Structure domains:
DNA-directed RNA polymerase subunit beta Chains: C, I
DNA-directed RNA polymerase subunit beta' Chains: D, J
Molecule details ›
Chains: D, J
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli BW2952
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit omega Chains: E, K
Molecule details ›
Chains: E, K
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
RNA polymerase sigma factor RpoD Chains: F, L
Molecule details ›
Chains: F, L
Length: 613 amino acids
Theoretical weight: 70.35 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00579 (Residues: 1-613; Coverage: 100%)
Gene names: JW3039, alt, b3067, rpoD
Sequence domains:
Structure domains:
Bacterial RNA polymerase inhibitor Chains: M, N
Molecule details ›
Chains: M, N
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P03704 (Residues: 1-64; Coverage: 100%)
Gene name: 2
Sequence domains: RNA polymerase inhibitor
Structure domains: RNA polymerase inhibitor

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 187.51Å b: 205.038Å c: 308.797Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.282
Expression system: Escherichia coli