› 4lln

X-ray diffraction
2.84Å resolution

Crystal structure of S. aureus MepR-DNA complex

Released:
DOI: 10.2210/pdb4lln/pdb
PDB entry 4lln coloured by chain, front view.
PDB entry 4lln coloured by chain, side view.
PDB entry 4lln coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural mechanism of transcription regulation of the Staphylococcus aureus multidrug efflux operon mepRA by the MarR family repressor MepR.
Birukou I, Seo SM, Schindler BD, Kaatz GW, Brennan RG
Nucleic Acids Res 42 2774-88 (2014)
PMID: 24293644

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117791 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH marR-type domain-containing protein Chains: A, B, C, D, I, J
Molecule details ›
Chains: A, B, C, D, I, J
Length: 140 amino acids
Theoretical weight: 16.26 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5Y812 (Residues: 2-139; Coverage: 99%)
Gene names: BN1321_80052, E1948_01710, E1948_06500, EP54_02880, EQ90_08705, FAF17_12965, G6W63_10695, G6Y24_06545, GO793_15615, GO814_10745, GO941_13505, GO942_09445, GQX37_11080, GZ156_06425, GZ163_02140, H2639_11915, M1K003_0421, NCTC10702_00659, NCTC13131_05788, NCTC5664_00388, NCTC7878_00347, NCTC7972_01611, SAMEA1531744_01160, SAMEA2078260_01697, SAMEA2078588_01240, SAMEA2080344_01299, SAMEA2081063_00451, SAMEA4008575_01717, SAMEA70146418_01252, SAMEA70153168_00678, mepR
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Palindromized mepR operator sequence Chains: E, F, G, H, K, L
Molecule details ›
Chains: E, F, G, H, K, L
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 76.343Å b: 76.231Å c: 109.41Å
α: 90.61° β: 104.75° γ: 106.27°
R-values:
R R work R free
0.208 0.206 0.241
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

MarR family transcription factors: dynamic variations on a common scaffold.
Deochand et al. (2017)
2 more

5 mentions without citation

MarR family proteins are important regulators of clinically relevant antibiotic resistance.
Beggs et al. (2020)
4 more