› 4lnc

Neutron Diffraction
2.19Å resolution

Neutron structure of the cyclic glucose bound Xylose Isomerase E186Q mutant

Released:
DOI: 10.2210/pdb4lnc/pdb
PDB entry 4lnc coloured by chain, front view.
PDB entry 4lnc coloured by chain, side view.
PDB entry 4lnc coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Primary publication:
Neutron structure of the cyclic glucose-bound xylose isomerase E186Q mutant.
Munshi P, Snell EH, van der Woerd MJ, Judge RA, Myles DA, Ren Z, Meilleur F
Acta Crystallogr D Biol Crystallogr 70 414-20 (2014)
PMID: 24531475

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.2 KDa
Source organism: Streptomyces rubiginosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

4 bound ligands:
Ligand GLC 1 x GLC
Ligand MN 2 x MN
Ligand MG 1 x MG
Ligand DOD 289 x DOD
No modified residues

Experiments and Validation Details

Spacegroup: I222
Unit cell:
a: 93.97Å b: 99.52Å c: 102.92Å α: 90° β: 90° γ: 90°
R-values:
R R work R free 0.288 0.284 0.322
Expression system: Escherichia coli

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Citations

3 review citations

Neutron protein crystallography: A complementary tool for locating hydrogens in proteins.
O'Dell et al. (2016)
2 more

2 mentions without citation

Evaluation of models determined by neutron diffraction and proposed improvements to their validation and deposition.
Liebschner et al. (2018)
1 more