› 4lng

X-ray diffraction
1.9Å resolution

Aspergillus fumigatus protein farnesyltransferase complex with farnesyldiphosphate and tipifarnib

Released:
DOI: 10.2210/pdb4lng/pdb
PDB entry 4lng coloured by chain, front view.
PDB entry 4lng coloured by chain, side view.
PDB entry 4lng coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Crystal structures of the fungal pathogen Aspergillus fumigatus protein farnesyltransferase complexed with substrates and inhibitors reveal features for antifungal drug design.
Mabanglo MF, Hast MA, Lubock NB, Hellinga HW, Beese LS
Protein Sci 23 289-301 (2014)
PMID: 24347326

Function and Biology Details

Reactions catalysed: 
Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate
Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175834 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 42.39 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WP27 (Residues: 1-353; Coverage: 100%)
Gene name: AFUA_4G07800
Sequence domains: Protein prenyltransferase alpha subunit repeat
Structure domains: Protein prenylyltransferase
Protein farnesyltransferase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 519 amino acids
Theoretical weight: 56.64 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WPS9 (Residues: 1-519; Coverage: 100%)
Gene name: AFUA_4G10330
Sequence domains: Prenyltransferase and squalene oxidase repeat
Structure domains: Glycosyltransferase

Ligands and Environments

5 bound ligands:
Ligand EDO 5 x EDO
Ligand ZN 1 x ZN
Ligand JAN 1 x JAN
Ligand FPP 1 x FPP
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 63.537Å b: 90.658Å c: 83.163Å
α: 90° β: 111.09° γ: 90°
R-values:
R R work R free
0.156 0.155 0.184
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Antifungals.
Campoy et al. (2017)
4 more

2 mentions without citation

Crystal structures of the fungal pathogen Aspergillus fumigatus protein farnesyltransferase complexed with substrates and inhibitors reveal features for antifungal drug design.
Mabanglo et al. (2014)
1 more