› 4lou

X-ray diffraction
2.98Å resolution

Structure of the E148Q mutant of CLC-ec1 deltaNC construct in the absence of halide

Released:
DOI: 10.2210/pdb4lou/pdb
PDB entry 4lou coloured by chain, front view.
PDB entry 4lou coloured by chain, side view.
PDB entry 4lou coloured by chain, top view.
Source organisms:
Primary publication:
A fluoride interloper in a CLC-type Cl /H+ antiporter
Lim H-H, Stockbridge RB, Miller C
Nat. Chem. Biol. (2013)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219743 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 446 amino acids
Theoretical weight: 47.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 16-460; Coverage: 94%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Fab heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab light chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 231.617Å b: 100.316Å c: 170.955Å
α: 90° β: 131.71° γ: 90°
R-values:
R R work R free
0.212 0.21 0.253
Expression systems:
  • Escherichia coli
  • Mus musculus

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