› 4lqs

X-ray diffraction
3.3Å resolution

Crystal structure of the Cbk1-Mob2 kinase-coactivator complex

Released:
DOI: 10.2210/pdb4lqs/pdb
PDB entry 4lqs coloured by chain, front view.
PDB entry 4lqs coloured by chain, side view.
PDB entry 4lqs coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism.
Gógl G, Schneider KD, Yeh BJ, Alam N, Nguyen Ba AN, Moses AM, Hetényi C, Reményi A, Weiss EL
PLoS Biol 13 e1002146 (2015)
PMID: 25966461

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155090 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase CBK1 Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 58.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53894 (Residues: 251-756; Coverage: 67%)
Gene names: CBK1, N1727, YNL161W
Sequence domains: Protein kinase domain
Structure domains:
CBK1 kinase activator protein MOB2 Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 28.42 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P43563 (Residues: 46-287; Coverage: 84%)
Gene names: MOB2, YFL034C-B, YFL035C, YFL035C-A
Sequence domains: Mob1/phocein family
Structure domains: MOB kinase activator

Ligands and Environments

1 bound ligand:
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 138.43Å b: 79.98Å c: 117.58Å
α: 90° β: 117.6° γ: 90°
R-values:
R R work R free
0.272 0.27 0.307
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Mechanisms of Hippo pathway regulation.
Meng et al. (2016)
9 more

4 mentions without citation

The Structure of an NDR/LATS Kinase-Mob Complex Reveals a Novel Kinase-Coactivator System and Substrate Docking Mechanism.
Gógl et al. (2015)
3 more