› 4lrv

X-ray diffraction
2.5Å resolution

Crystal structure of DndE from Escherichia coli B7A involved in DNA phosphorothioation modification

Released:
DOI: 10.2210/pdb4lrv/pdb
PDB entry 4lrv coloured by chain, front view.
PDB entry 4lrv coloured by chain, side view.
PDB entry 4lrv coloured by chain, top view.
Source organism: Escherichia coli B7A
Primary publication:
Structural insights into DndE from Escherichia coli B7A involved in DNA phosphorothioation modification.
Hu W, Wang C, Liang J, Zhang T, Hu Z, Wang Z, Lan W, Li F, Wu H, Ding J, Wu G, Deng Z, Cao C
Cell Res 22 1203-6 (2012)
PMID: 22525332

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA sulfur modification protein DndE Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 109 amino acids
Theoretical weight: 12.57 KDa
Source organism: Escherichia coli B7A
Expression system: Escherichia coli
Structure domains: DNA sulphur modification protein DndE

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 97.845Å b: 97.306Å c: 218.532Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.203 0.201 0.242
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Evolutionary Genomics of Defense Systems in Archaea and Bacteria.
Koonin et al. (2017)
3 more

3 mentions without citation

Knotting and unknotting proteins in the chaperonin cage: Effects of the excluded volume.
Niewieczerzal et al. (2017)
2 more