› 4lsj

X-ray diffraction
2.35Å resolution

Crystal Structure of the Glucocorticoid Receptor Ligand Binding Domain Bound to a Dibenzoxapine Sulfonamide

Released:
DOI: 10.2210/pdb4lsj/pdb
PDB entry 4lsj coloured by chain, front view.
PDB entry 4lsj coloured by chain, side view.
PDB entry 4lsj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Glucocorticoid receptor modulators informed by crystallography lead to a new rationale for receptor selectivity, function, and implications for structure-based design.
Carson MW, Luz JG, Suen C, Montrose C, Zink R, Ruan X, Cheng C, Cole H, Adrian MD, Kohlman DT, Mabry T, Snyder N, Condon B, Maletic M, Clawson D, Pustilnik A, Coghlan MJ
J Med Chem 57 849-60 (2014)
PMID: 24446728

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137444 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucocorticoid receptor Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04150 (Residues: 522-777; Coverage: 33%)
Gene names: GRL, NR3C1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
D30 peptide Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.57 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand LSJ 2 x LSJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P21212
Unit cell:
a: 38.962Å b: 139.398Å c: 48.068Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.18 0.236
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Mechanisms of NF-κB p65 and strategies for therapeutic manipulation.
Giridharan et al. (2018)
8 other articles

3 mentions without citation

The Biologist's Guide to the Glucocorticoid Receptor's Structure.
Deploey et al. (2023)
2 more