› 4ltb

X-ray diffraction
2.59Å resolution

Coiled-coil domain of TRIM25

Released:
DOI: 10.2210/pdb4ltb/pdb
PDB entry 4ltb coloured by chain, front view.
PDB entry 4ltb coloured by chain, side view.
PDB entry 4ltb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The tripartite motif coiled-coil is an elongated antiparallel hairpin dimer.
Sanchez JG, Okreglicka K, Chandrasekaran V, Welker JM, Sundquist WI, Pornillos O
Proc Natl Acad Sci U S A 111 2494-9 (2014)
PMID: 24550273

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin/ISG15 ligase TRIM25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14258 (Residues: 189-379; Coverage: 30%)
Gene names: EFP, RNF147, TRIM25, ZNF147

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 57.669Å b: 83.158Å c: 92.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.203 0.257
Expression system: Escherichia coli

Quick links

Citations

19 review citations

RIG-I-like receptors: their regulation and roles in RNA sensing.
Rehwinkel et al. (2020)
18 more

13 mentions without citation

HIV suppression by host restriction factors and viral immune evasion.
Jia et al. (2015)
12 more